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KMID : 0903519890320020085
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1989 Volume.32 No. 2 p.85 ~ p.90
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Two - Dimensional Electrophoretic Analysis of Rice Seed Proteins
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Abstract
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High resolution two-dimensional (2-D) electrophoresis with isoelectrofocusing in the first dimension and electrophoresis in sodium dodecyl sulfate in acrylamide gradient gels in the second dimension has been used to produce maps of proteins, extracted from rice seeds with 2% sodium dodecyl sulfate/5% 2-mercaptoethanol. Six rice cultivars-three Japonica types and three Tongil(high-yielding) types-at six maturities were studied. Composite map was constructed and more than 300 polypeptide spots were counted in the pH range of 5.2∼8.3 and molecular range of 20,000∼100,000. Vast differences were observed between varieties and between maturities in the maps.
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